ꆪ훐맺쪳욷컀짺퓓횾44ꆪCHINESEJOURNALOFFOODHYGIENEꆡꆡꆡꆡꆡꆡꆡꆡ2007쓪뗚19뻭뗚1웚싛훸쪳욷훐샮쮹쳘뻺컛좾ힴ뿶퇐뺿럫볒췻ꆡ컢킡싗ꆡ돂뺲뺲ꆡ췵킡폱ꆡ샮떤쇕ꆡ쳆쪳쏷ꆡ폎쫧훩(훩몣돶죫뺳볬퇩볬틟뻖,맣뚫훩몣ꆡ519015)햪ꆡ튪:쒿뗄ꆡ쇋뷢샤님샤뚳쪳욷훐샮쮹쳘뻺컛좾ힴ뿶ꆣ랽램ꆡ닉폃PCR랽램뛔퇹욷훐뗄샮쮹쳘뻺뷸탐돵즸,퇴탔퇹욷폃맺뇪램좷횤,뛔6샠샤님샤뚳쪳욷릲3586럝퇹욷뷸탐쇋샮쮹쳘뻺듸뻺ힴ뿶뗷닩ꆣ뷡맻ꆡ훩몣뗘쟸샮쮹쳘뻺컛좾뷏캪웕뇩,쟒듦퓚ퟅ뛠훘컛좾쿖쿳,짺쿊쓌ꆢ짺죢쟝샠ꆢ쫟닋샠퇹욷늻뷶듸샮쮹쳘뻺싊룟,쟒듸떥퓶샮쮹쳘뻺싊룟ꆣ뷡싛ꆡ펦볓잿샤뚳쪳욷뗄샮쮹쳘뻺볠닢폫맜샭ꆣ맘볼듊:샻쮹쳘쫏뻺쫴;샻쮹쳘쫏뻺,떥뫋쾸냻퓶짺;쪳욷컛좾InvestigatigonofContaminationStatusofFoodsbyListeriainZhuhaiFENGJia2wang,WUXiao2lun,CHENJing2jing,WANGXiao2yu,LIDan2ling,TANGShi2ming,YOUShu2zhu(,GuangdongZhuhai519015,China)Abstract:ObjectiveꆡꆡInthesummermonthsofrecantyears,,andthenthenationalstandardmethod,GBΠT4789130ꆪ2003,ꆡTheresultsshowedthattherewereheavycontaminationoffoodsbyListeriainZhuhai,,rawmeat,ꆡ:Listerial;Listeriamonocytogenes;FoodContaminationꆡꆡ샮쮹쳘뻺쫴릲폐7룶훖,벴떥뫋쾸냻퓶짺탔샮쇏ꆢ얣쓌ꆢ죋뫍뚯컯뗄럠뇣훐뗈,룃뻺죝틗컛좾쪳욷쮹쳘뻺(Listeriamonocytogenes,틔쿂볲돆떥퓶샮쮹쳘틽웰죋뗄쪳컯훐뚾,쫇죋탳릲뮼뗄훘튪쪳풴탔훂늡뻺)ꆢ쒬쫏샮쮹쳘뻺()ꆢ컷뛻샮쮹쳘뻺(L.뻺,놻WHO쇐캪20쫀볍90쓪듺쪳욷훐4듳훂늡뻺seeligeri)ꆢ룱쫏샮쮹쳘뻺()ꆢ췾뛻쮹샮쮹쳘횮튻[1]ꆣ뷼쓪살,떥퓶샮쮹쳘뻺퓚램맺ꆢ쏀맺ꆢ볓쓃뻺()ꆢ쏠퇲샮쮹쳘뻺()ꆢ펢억듳뗈맺퓸뛠듎틽웰쪳컯훐뚾[2,3],쟡헟캪튻냣캸뎦퇗뿋샮쮹쳘뻺(),웤훐떥퓶샮쮹쳘뻺뫍쏠횢ힴ,훘횢헟훷튪뇭쿖캪냜톪횢ꆢ쓔쒤퇗ꆢ짱뺭횢ힴ퇲샮쮹쳘뻺폐훂늡탔,떥퓶샮쮹쳘뻺뗄훂늡솦뷏잿ꆣ벰떥뫋쾸냻퓶뛠뗈,쇙뒲쯀췶싊룟듯20%ꆫ떥퓶샮쮹쳘뻺쫇튻훖룯삼퇴탔컞톿볦탔퇡퇵70%[4,5],틽웰쇋쫀뷧룷맺뗄룟뛈맘힢ꆣ뛌킡룋뻺,맣랺듦퓚폚ퟔ좻뷧,죧췁죀ꆢ컛쮮ꆢ쟠훼쯇떥퓶샮쮹쳘뻺쓜퓚(4ꇀ2)ꇦ뮷뺳훐짺뎤ꆢ랱횳,ꆡꆡꆡꆡ[4]ꆡZANGJINGCUN,[6]ꆡTAKEDAT,YAMAGATAK,YOUHIDAY,[J].HighTechnologyimmunochromatographybasedrapiddetectionkitforfecalLetters,1996,3:[J].KansenshogakuZasshi,1998,72(8):8342[5]ꆡNIEDBALARS,FEINDTH,KARDOSK,[J].[쫕룥죕웚:2006-10-20]AnalyticalBiochemistry,2001,293:22230.훐춼럖샠뫅:R15;R117;R378121ꆡꆡ컄쿗뇪쪶싫:Aꆡꆡ컄헂뇠뫅:1004-8456(2007)01-0041-04믹뷰쿮쒿:맺볒훊솿볠뚽볬퇩볬틟ퟜ뻖뿆퇐쿮쒿(2002IK002-08)ퟷ헟볲뷩:럫볒췻ꆡ쓐ꆡ룟벶릤돌쪦
쪳욷훐샮쮹쳘뻺컛좾ힴ뿶퇐뺿ꆪꆪꆪ럫볒췻ꆡ컢킡싗ꆡ돂뺲뺲뗈ꆪ45ꆪ늢쓜쓍쫜-20ꇦ뗄뗍컂쟒늿럖듦믮,틲뛸뎣컛좾샤놣님훐탄,웤훐떥퓶샮쮹쳘뻺1훪,샮쮹쳘뻺쫴웤쯻님ꆢ샤뚳쪳욷벰냍쫏쿻뚾쓌,틽웰쪳컯훐뚾ꆣ룷훖샤6훖뻺룷1훪,뎦퇗즳쏅뻺(Salmonellaenteritidis)1님ꆢ샤뚳쪳욷컛좾킯듸샮쮹쳘뻺뗄쟩뿶닮틬뷏듳,캪훪,십뫬쟲뻺(Rhodococcusequi)1훪ꆣ쇋쓜룼뿆톧뗘풤럀쪳풴탔샮쮹쳘뻺훐뚾,뛔듸뻺쪳ꆡ엠퇸믹벰틇웷짨놸ꆡ엠퇸믹뻹릺ퟔ붭쯕틋욷뷸탐뗷닩럖컶뇘늻뿉짙,놾퇐뺿뛔훩몣뗘쟸샤뚳탋폀탅캢짺컯쫔볁뎧;캢탍짺뮯맜릺ퟔ놱뺩슽쟅릫짺죢(냼삨쟝샠)ꆢ샤뚳짺쮮닺욷ꆢ샤님짺쟝ꆢ샤뚳샤쮾;뿆싪볎샮쮹쳘뻺쿔즫엠퇸믹릺ퟔ횣훝늩주짺컯님뚹훆욷ꆢ샤뚳샤님쫟닋ꆢ쿊쓌6샠쪳욷릲3586럝릫쮾;APIListeria짺뮯본뚨쫔볁쳵평램맺짺컯쎷샯퇹욷훐뗄듸뻺쟩뿶뷸탐쇋뗷닩럖컶ꆣ낣릫쮾짺닺;Microscan캢짺컯럖컶틇AutoScan24탍캪쏀맺Microscan릫쮾닺욷,룷훖뫄닄ꆢ룯삼퇴탔ꆢ틵1ꆡ닄쇏폫랽램탔랴펦냥뻹캪짏몣뗂쇩(Microscan)릫쮾쳡릩ꆣꆡ닄쇏ꆡ랽램ꆡPCR틽컯ꆢ쫔볁벰틇웷짨놸뗈ꆡ닎볻닎뾼컄ꆡ돵즸랽램ꆡ닉폃PCR랽램뛔퇹욷뷸탐샮쮹쿗[6,7]ꆣ쳘뻺돵즸,뻟쳥랽램닎헕닎뾼컄쿗[6,7]ꆣꆡ쪳욷닄쇏ꆡ훷튪릺ퟔ훩몣쫐룷듳뎬쫐,닉벯ꆡ좷횤랽램ꆡ돵즸캪퇴탔뗄쫔퇹,닉폃GBΠT쪱볤뻹캪쎿쓪뗄쿄벾(6-9퓂),릲닉벯3586럝퇹4789130ꆪ2003뗄맺뇪램[8]뷸탐좷뚨,웤훐뗄짺뮯랴욷,럖캪6샠ꆣ펦닉폃캢탍짺뮯맜ꆢAPIListeria짺뮯본뚨쫔볁쳵뫍샤뚳짺죢ꆢ쟝샠ꆡ냼삨뚳훭업맇ꆢ훭뷅ꆢ훭짠ꆢ훭캢짺컯럖컶틇뗄럖컶뷡맻ꆣ뛇;뚳벦ꆢ벦틭ꆢ벦췈ꆢ벦ꆣ샤뚳짺쮮닺욷샠ꆡ냼삨뚳쾺ꆢ쾺죊ꆢ폣ꆢ쒫폣ꆢ2ꆡ뷡맻폣ꆢ폣욬ꆢ식죢ꆢ폣돡ꆣꆡ떥퓶샮쮹쳘뻺듸뻺쟩뿶ꆡ릲볬닢3586럝퇹샤님짺쟝샠ꆡ냼삨샤님벦ꆢ톼ꆢ룫ꆣ욷,떥퓶샮쮹쳘뻺퇴탔퇹욷78룶,퇴탔퇹욷싊샤뚳샤님뚹훆욷ꆡ냼삨샤뚳믲샤님뛠캶뚹뢯2118%;웤훐뇹쿊폣돡듸뻺싊ퟮ룟,캪36136%;웤듎샠ꆢ벦죢뚹뢯냼Π뻭샠ꆢ쫟닋뚹뢯냼Π뻭샠ꆢ폍햨뚹뢯캪짺쿊쓌ꆢ뚳훭뛇ꆢ뚳훭짠ꆢ뚳훭뷅,듸뻺싊10%ꆫ샠ꆢ쓪룢뚹뢯냼Π뻭샠ꆣ15%;샤님벦ꆢ톼ꆢ룫퓚6%ꆫ8%;웤쯼뚳쮮닺욷ꆢ샤샤뚳샤님쫟닋샠ꆡ냼삨샤뚳믲샤님쟠릣닋ꆢ듳님죢쟝샠ꆢ샤뚳쫟닋샠듸뻺싊뷏뗍ꆣ샤님쫟닋샠듸냗닋ꆢ시늷ꆢ쯄벾뚹뗈ꆣ뻺싊캪2133%,놣쿊얣쓌듸뻺싊캪0168%,횵뗃힢쿊쓌ꆡ냼삨짺쿊쓌ꆢ냍쫏쿻뚾놣쿊얣쓌ꆣ짺쿊틢ꆣ뚹훆욷샠틲돩볬뗄뻹캪냫돉욷,캴랢쿖듸뻺쟩쓌닉ퟔ얣쓌퇸횳뎡믲릩냄쏅뗄풭쇏쓌ꆣ뿶(쿪볻뇭1)ꆣꆡ뇪ힼ뻺훪ꆡ릲9훖,뻹릺ퟔ훐맺뗤탍엠퇸컯ꆡ웤쯻샮쮹쳘뻺듸뻺쟩뿶ꆡ퓚6샠쪳욷릲뇭ꆡꆡ1ꆡ6샠쪳욷훐떥퓶샮쮹쳘뻺듸뻺쟩뿶뇭샠뇰쏻돆퇹욷쫽훼듦랽쪽퇴탔퇹욷쫽퇴탔퇹욷싊(%)샤뚳짺죢ꆢ쟝샠훭업맇112샤뚳훭뷅87샤뚳훭짠32샤뚳훭뛇47샤뚳벦37샤뚳벦틭25샤뚳벦췈6샤뚳00100벦뷅19샤뚳톼24샤뚳룫14샤뚳00100샤뚳짺쮮닺욷샠폣ꆢ폣욬158샤뚳00100폣ꆢ쒫폣80샤뚳00100쾺ꆢ쾺죊210샤뚳쿊폣돡11샤뚳식죢24샤뚳샤님짺쟝샠벦23샤님톼31샤님룫35샤님샤뚳Π샤님뚹훆욷뚹뢯샠1328샤뚳Π샤님00100샤뚳Π샤님쫟닋샠샤뚳쫟닋샠506샤뚳샤님쫟닋샠558샤님쿊쓌놣쿊얣쓌147샤님짺쿊쓌72샤님뫏볆
ꆪ46ꆪ훐맺쪳욷컀짺퓓횾CHINESEJOURNALOFFOODHYGIENEꆡꆡꆡꆡꆡꆡꆡꆡ2007쓪뗚19뻭뗚1웚3586럝퇹욷훐,돽떥퓶샮쮹쳘뻺췢,뮹볬돶룱쫏ꆢ췾ퟮ룟,캪45183%,웤듎캪샤님ꆢ샤뚳짺죢쟝샠,럖뇰뛻쮹ꆢ쒬쫏ꆢ펢억뿋4훖샮쮹쳘뻺,퇴탔싊럖뇰캪캪13148%ꆢ4171%,쫟닋샠캪1197%ꆣ퇹욷듦퓚뛠1153%ꆢ1114%ꆢ0195%뫍0173%,ퟜ뗄퇴탔퇹욷싊훘컛좾,춬튻퇹욷훐듸2훖틔짏샮쮹쳘뻺뗄쟩뿶뷏캪2148%,웤훐짺쿊쓌훐웤쯻샮쮹쳘뻺퇴탔퇹욷싊캪웕뇩(쿪볻뇭2)ꆣ뇭2ꆡ웤쯻샮쮹쳘뻺듸뻺쟩뿶뇭퇹욷샠뇰퇹욷쫽룱쫏샮췾뛻쮹쒬쫏샮펢억뿋퇴탔퇹퇴탔퇹쮹쳘뻺샮쮹쳘뻺쮹쳘뻺샮쮹쳘뻺욷쫽욷싊(%)샤뚳짺죢ꆢ쟝샠샤뚳짺쮮닺욷샠샤님짺쟝샠샤뚳Π샤님뚹훆욷1328000000100샤뚳Π샤님쫟닋샠쿊쓌샠(짺쿊쓌)쿊쓌샠(놣쿊얣쓌)147000000100뫏볆퇴탔싊(%)ힴ뿶폅폚닋쫐뎡,닋쫐뎡뗄듸뻺싊뿉쓜룼룟ꆣ뗷닩3ꆡ뷡싛뫍쳖싛뗄샤뚳샤님뚹훆욷쫇틑볓릤쿻뚾맽뗄냫돉욷,웤듸놾퇐뺿뷡맻쿔쪾,6샠샤님샤뚳쪳욷훐,떥퓶샮뻺쟩뿶늻쓜듺뇭웕춨쫐쫛뚹훆욷샠ꆣ듋듎퇐뺿훐,쮹쳘뻺퇴탔퇹욷싊캪2118%,웤쯻샮쮹쳘뻺퇴탔퇹캴볬돶쏠퇲샮쮹쳘뻺뫍컷뛻샮쮹쳘뻺,뻟쳥풭틲죔욷싊캪2148%,뛠훘컛좾쿖쿳뷏캪웕뇩,짺쿊쓌ꆢ짺퓚뗷닩럖컶횮훐ꆣ죢쟝샠ꆢ쫟닋샠늻뷶듸샮쮹쳘뻺싊룟,쟒듸떥퓶샮쮹쳘뻺싊룟,쫇훷튪뗄캣쿕쪳욷ꆣ닎뾼컄쿗짺쿊쓌쫇쾸뻺랱횳뗄솼뫃믹훊,웤벷쓌ꆢ퓋쫤뮷[1]ꆡRYSERT,[J].Marcel뷚뗄샤솴쳵볾컞램폐킧틖훆샮쮹쳘뻺뗄랱횳,맊웤DekkeInc,1991,6(2):2142216.듸샮쮹쳘뻺벰떥퓶샮쮹쳘뻺뗄뇈싊뷏룟[2]ꆡSCHLECHWF,LAVIGNEPM,,떥퓶샮쮹쳘뻺뻟폐튻뚨뗄죈뗖뾹쓜솦Listeriosis:evidenceofTransmissionbyfood[J].NJMed,1983,,쓍쫜냍쫏쿻뚾,퓚308:ꇦ쿂탨20min믲70ꇦ쿂탨5min닅쓜즱쯀,튻떩[3]ꆡ쇵탣럥,돂욽,컢뮤뮪.떥뫋쾸냻퓶짺탔샮쮹쳘뻺늡퇐뺿뷼뿶짺쿊쓌냍쫏즱뻺늻뎹뗗,뻍폐평쪳풴탔틽웰떥퓶샮[J].쪵폃풤럀튽톧,2001,8(1):79281.쮹쳘뻺늡놩랢뗄캣쿕ꆣ[4]ꆡ[J].ClinIntectDis,1997,24:1211.죢샠뗄뢱닺욷,죧훭뛇ꆢ훭짠ꆢ훭뷅뗈뗄떥퓶샮[5]ꆡ폴쟬뢣.쿖듺컀짺캢짺컯톧[M].놱뺩:죋쏱컀짺돶냦짧,쮹쳘뻺듸뻺싊쏷쿔룟폚짺죢쟝샠,뿉쓜쫇평폚웤쫇1995,119.[6]ꆡ럫볒췻,컢킡싗,믆퓆뻽,뗈.쪳욷훐떥뫋퓶짺탔샮쮹쳘뻺뗄뢱닺욷,뷏틗컛좾,쟒컀짺ힴ뿶늻쫜훘쫓믲뷏쓑뿘훆PCR뿬쯙볬닢퇐뺿[J].훐맺쪳욷컀짺퓓횾,2005,3(17):2342뗄풭틲ꆣ237.쫟닋샠뗄떥퓶샮쮹쳘뻺듸뻺싊캪1150%,듸웤[7]ꆡANDREASBUBERT,STEPHANK;HLER,WERNERGOEBEL.쯻샮쮹쳘뻺뗄퇴탔퇹욷싊캪Thehomologousandheterologousregionswithintheiapgeneallow1197%,짺돔쫟닋퓸퓚볓쓃듳ꆢ쏀맺뗈틽훂떥퓶샮쮹쳘뻺늡뗄놩랢쇷[J].ApplEnvironMicrobiol,1992,58(8):262522632.탐[2,3],떼훂뛠죋쯀췶,컒맺쓏랽뗘쟸폐짺돔솹냨닋ꆢ[8]ꆡGBΠꆪ2003.쪳욷컀짺캢짺컯톧볬퇩떥뫋쾸냻퓶짺샮즳삭ꆢ냫쫬쟠닋뗄쾰맟,뿍맛뮷뺳쳵볾샻폚쪳풴탔떥쮹쳘쫏뻺볬퇩[S].퓶샮쮹쳘뻺늡뗄랢짺쇷탐,펦틽웰룟뛈훘쫓ꆣ[쫕룥죕웚:2006-10-20]놾퇐뺿뗄퇹욷뻹살ퟔ훩몣쫐룷듳뎬쫐,웤컀짺훐춼럖샠뫅:R15;R117;;ꆡ컄쿗뇪쪶싫:Aꆡ컄헂뇠뫅:1004-8456(2007)01-0044-03
